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100x Multiplex T-Cell Activation

Discover the power of our cutting-edge 100x Multiplex T-Cell Activation technique. This innovative approach transforms T-cell activation experiments by using a cyclic design to repeatedly test and collect data from a single sample. 

Experience a dramatic boost in data yield, amplifying your results. 

The 100XBIO Multiplex T-cell activation process involves multiple cycles of antigen-specific stimulation to analyze T-cell responses. Initially, T cells are pre-treated and plated. In the first cycle, peptide antigen #1 is added, and a fluorescent readout identifies activated T cells. The process is repeated in subsequent cycles, with different peptide antigens introduced each time, allowing for extensive analysis of T-cell activation. This method can be performed for up to 100 cycles, with the diagram illustrating the progression from untreated T cells through peptide stimulation to fluorescence-based activation detection.

The 100XBIO Multiplex T-cell activation process involves multiple cycles of antigen-specific stimulation to analyze T-cell responses. This method can be performed for up to 100 cycles, with the diagram illustrating the progression from untreated T cells through peptide stimulation to fluorescence-based activation detection.

An Unusual Cyclic Experiment Design

Unleashing 100x Data Amplification

Welcome to the future of data enhancement with our innovative cyclic approach. Our innovative method amplifies your data by a staggering 100-fold, unlocking new possibilities for research and discovery.

Dynamic Cyclic Testing

Our process begins with a sensor-pretreated sample on a membrane. Through a series of rapid, 10-minute cycles, each cycle introduces a new target while removing the old one. This dynamic testing environment allows you to evaluate up to 100 targets one by one.

Sample 

Multiple Cycles (Target Testing & Data Collection)

Enhanced Data

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Replace the old target with a new one

100X increase in data

Response to each new target

Unmatched Data Output

By leveraging this cyclic approach, you experience a dramatic increase in data output—up to 100 times more data than traditional methods. Each response to a new target is assessed by excluding earlier activated cells from analysis.

Precision Confirmation

To validate each response, we can employ surface staining and intracellular staining for cytokines and phenotypic markers, including activation-induced markers (AIM). This ensures that every data point is accurately confirmed, providing you with reliable and actionable insights.

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