Introducing AIMSpot
AIMSpot offers a novel approach to T-cell activation analysis. By seamlessly integrating automated live cell assays with antibody staining cycles, AIMSpot eliminates non-specific activation signals, enabling a deep dive into the intricate phenotypes, activation markers, and cytokine secretion profiles of T-cells.
Industry Standards vs. 100XBIO: A Comparative Analysis
ELISpot/FluoroSpot, Flow Cytometry (FACS-ICS/FACS-AIM), and AIMSpot are techniques used to analyze cytokine-secreting cells and T-cell responses.
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The image compares three methods for antigen-specific T-cell response: ELISpot/FluoroSpot, Flow cytometry-ICS/AIM, and AIMSpot.
AIMSpot involves multiple cycles of T-cell activation, with each cycle lasting 10 minutes (subject to further optimization) and gentle diffusion-driven washes. Post-activation the cells can undergo cycles of antibody staining. This method projects to provide 100 times more data on antigen-specific activation per sample, and allows to study cell phenotypes using image cytometry.