Hybrid Cytometry
seeing new dimensions

Hybrid Cytometry is a new fully automated research method that brings together the tracking of live-cell dynamics and an analysis of multiple makers of the cells stained post fixation. Our aim is to add a new meaningful data layer, and automate complex 2-day cell-based experiments. Would you like to watch a demo video? Please fill out the request form.

The principles of Hybrid Cytometry and the optimal designs of instruments and consumables are the intellectual property of 100XBIO. At a high level we integrated 3-color fluorescence microscopy inside the liquid handler, and engineered the method for gentle liquid exchange around the cells (even non-adherent) without centrifugation in the special 96-well plates.

We have created this system to automate standard cell-based experiments that require cell culture incubation with treatments for up to 48 hours. Our system would maintain the cell culture at 37 °C within the incubated enclosure, perform the programmed treatments and track the live cells taking photographs up to 4-6 times per hour in a full-plate format.

The system can lower the temperature to RT and perform automated staining of non-fixed cells, fixation, permeabilization and staining of fixed cells. The fixed-cell staining can be run in multiple rounds. The system would acquire the fixed-cell data, connecting them with the live-cell history. The data can be presented in flow-cytometry-like formats.

Our system’s applications include targeted delivery, GFP expression, cytotoxicity, killing, and proliferation assays. Unlike current approaches, where dynamic cellular responses or gene expression would be studied with live-cell imaging, and phenotypes would be analysed using flow cytometry, our system allows one automated assay for both readouts combined.

The main advantages of the system come from the Hybrid single-cell data, and from the gentle liquid exchange without centrifugation. We can look at cells responding sequentially to diverse stimuli, study cell-cell contacts, dynamic cell death and killing. Gentle liquid exchange is great for studying precious, rare and unique samples, and is great for sensitive or fragile cell types.

We are the quantitative live-cell and fixed-cell imaging platform that allows the users to automate immunofluorescence (IF) staining or immunocytochemistry (ICC) protocols for the cell culture, combined with automated image acquisition of the plate. We’re also automating the time-lapse treatments for the live-cell experiments, and data analysis workflows.

We begin with an exploratory call and a brief 10-week service project that allows both parties to validate the system’s usefulness for the specific application. It is a paid project, but we are discounting its cost to stay below the market price for similar efforts, accounting for the risks involved. If you like the generated data, we will explain the further demo or purchasing options.

As our technology evolves, our earlier publications have become outdated. Please refer to the latest abstracts and posters on our website (this section is coming soon). We share our white paper upon request via the contact form.

We will host regular webinars for our current and prospective users. They will be normally hosted at the end of the month, the next meeting is on April 28th. If you’d like to receive an invitation to the next meeting, request this via the contact form. In the same form you can request access to the latest recording, kept as an unlisted video at our YouTube channel.